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quote:

Originally posted by KiNeTiC ENeRgY Good deal. It's tough to get anywhere in science w/o a phd anymore, and the money is usually garbage to boot.

Yeah if you're retarded and don't realize that you have huge fucking claws and fangs and that if you work for yourself you will make at least 100,000/year to upwards of tens of millions once the heavy hitters come around trying to buy your company.

x^-1 = 1/x

ml^-1 = 1/ml

It's another way of writing something (for example) as 5,000/ml (as in 5,000 units per ml). Instead you'd write 5,000 ml^-1.

Re: Any scientists/mathmaticians? Quick question

quote:

Originally posted by Jackson Would it be nanolitres? centilitres?

http://en.wikipedia.org/wiki/Litre#...ed_to_the_litre

Wikipedia knows what nanolitres and centilitres are. They just don't happen to be part of the answer you're looking for

quote:

Originally posted by NeoPhono x^-1 = 1/x ml^-1 = 1/ml It's another way of writing something (for example) as 5,000/ml (as in 5,000 units per ml). Instead you'd write 5,000 ml^-1.

This. Doing the calculations you get the number of cells per volume of culture, that is cells/ml = cells x ml-1 = 7500 ml-1

Here's an example of it being used (although it's still much more common to see x/ml than x ml-1).

http://www.tripdatabase.com/spider.html?itemid=630762

quote:

Antiemetic effect of ondansetron 0.2 mg mL-1 in PCA morphine solution.

Which is the same as "0.2 mg/mL"

EDIT: This is probably a more fitting example;

http://www.natureprotocols.com/2008...spheres_fro.php

From the "Proceedure" section;

quote:

Plate the single-cell ES cell suspension in 50 ml fresh EB medium at a density of 105 cells ml-1 onto 15-cm bacterial dishes.

There are more examples there as well...

yeah ml^-1 is units per mil just like s^-1 = hz

quote:

Originally posted by Azz3D 1/ml

Ahh Still stuck on this bullshit.

Im just gonna write down all the info i have:
Ok the basic experiment is to work out the viability of a cuture of S. cerevisiae

We have already calculated the total count as 7.5x10^7 (or 75,000,000)

Now i'm calculating the Viable count (number of living cells).
I have calculated the average number of colonies in a 10^-4 dilution and a 10^-5 dilution.

In the 10^-4 dilution there were 165 colonies in agar.
So per ml I work this out to be 1.65x10^6 (correct?)

In the 10^-5 dilution there were 10 colonies in agar.
So per ml I work this out to be 1.0x10^6 (correct??)

The average of these two figures is 1.325x10^6 (or 1,325,000)

Percentage viability of a culture = viable cells divided by total cells x 100%

So 1.325x10^6 over 7.5x10^7 x100 = 1.77%

This seems very low to me, and i think i've missed a zero out somewhere beacause 17.7% would be more believable.

Any ideas???

Thanks everyone for this, its just I need to get past this to complete the rest of the work.

Cheers

Re: NEW - Any scientists/mathmaticians? Quick question

quote:

Originally posted by Jackson I've got a serious mental block and cant work this out. What does ml-1 equal? I am not too bad with scientific notation since we've just come onto it. But I can't work it out...would it be nanolitres? centilitres?

technically 1*1 = 1 right

so 1 * ml = ml or 1ml

So you have (1ml)^-1 = (1)/(1ml)

and 1ml = 1*10^-3 L = .001L

so you have (1ml)^-1 = (1)/(1ml) = (1)/(.001L)

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