You can have two bass sequences playing back at the same time. As a matter of fact you can make any arrangemments in your track as long as the kick and the subbass are not clashing. Therefore, keep the subbass on a separate beat from the kick, and play the midbass to give it the groove.

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Sub-bass can be added on the kick. Try this. Use the sub-bass on the offbeat and when it hits on the kick use a processed version of that same sub-bass. You should use a program that emphasizes the upper harmonics of the bass on that note, while deemphasizing the lower frequencies. This can really add to the groove when you don't have to worry about putting the subbass on offbeat notes.

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