Try changing your audio driver. I had the same problem once... after desperately tweaking everything, switching my audio driver to ASIO made the file accessible. Go figure. I resaved the file and switched back to my US-122 primary driver.
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One always has to remember these days where the garbage pail is, because it's so easy to make sounds, and to put sounds together into something that appears to be music, but it's just as hard as it always was to make good music.
- Robert Moog
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