If anyone here's ever done any Flow Cytometry, please give me a yell. I'm in dire need of assistance.

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quote:

Originally posted by Trance Nutter If anyone here's ever done any Flow Cytometry, please give me a yell. I'm in dire need of assistance.

haha what the hell is that?

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quote:

Originally posted by Philby haha what the hell is that?

the most barstardised experiment ever.

I hate it with a passion. It should be a piece of piss, but it never works.

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**Man I'm Pretty**

something to do with cells and biology?

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quote:

Originally posted by Philby something to do with cells and biology?

good guess

basically the idea is that you label cells using particular dyes. Each dye represents somthing different, eg one represents a dead cell, so any detection of this dye and you know the cell is dead. The cells are passed one at a time in a liquid stream (ie flow) past a detector (spectrometer), which lasers them and picks up the dye signal, hence telling you the proprtion of cells with each dye.

Sounds easy.

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ok well ive got this:
----|-*-/
....*...*
----|-/
okay so its a crazy triangular circuit right with the * representing resistors and i need to get the total resistance im confused what you use:
series = Rt = R1 + R2 + R3
OR
parallel = 1/Rt = 1/R1 + 1/R2 + 1/R3

or a mixture

edit: pretend the .'s arent there it screws up with out them the diagram lol

Last edited by Dj_Es-Dva on Oct-27-2005 at 12:11

from the way u drew that picture it looks like there are 2 resistors in series and 1 parallel to it so maybe do it like that...but don't rely on just me.....

quote:

Originally posted by Trance Nutter Sounds easy.

but.....?

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Break up the problem into parts that are in series and work out total resistance there (like mini-circuits), then you will have a few different "subtotal" resistances that are in parallel.

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Two Tribes '06 - Sunday March 5 @ Supreme Court Gardens
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My references section is about 10% of the length of the actual essay lol

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Two Tribes '06 - Sunday March 5 @ Supreme Court Gardens
PM for more info

quote:

hey do any of you find it interesting reading old journal papers on topics that are now commonly accepted but then were not widely known or accepted? i'm reading a paper by wilson called "continental drift" which was published before plate tectonics was established, and i think its really interesting to read about ideas and concepts that are now taken for granted or assumed but then were either controversial ideas or not even invented/existed yet! ie now everybody knows (well i mean like everyone doing geology) that the plates (they didn't have plates then, just continents!) move around and we had supercontinents and stuff, but at the time people talking about stuff like that were laughed at and told "get stuffed thats prepostorous". anyways i think its pretty interesting...

i have got a book called standing on the shoulders of giants, and it basically has the theories of copernicus, kepler, newton and einstein. i also did a subject called the history and philosophy of astronomy, which studied texts from ancient greece up until copernicus and some of the things that are written if you take them in context with the time they were written are incredible. although the language that they use is very bizarre and hard to understand.

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ATTORNEY: Now doctor, isn't it true that when a person dies in his sleep,
he doesn't know about it until the next morning?
WITNESS: Did you actually pass the bar exam?

quote:

Originally posted by Teflon_Teapot i have got a book called standing on the shoulders of giants, and it basically has the theories of copernicus, kepler, newton and einstein. i also did a subject called the history and philosophy of astronomy, which studied texts from ancient greece up until copernicus and some of the things that are written if you take them in context with the time they were written are incredible. although the language that they use is very bizarre and hard to understand.

haha yeah it would have been crazier back then, ie "the earth is not the centre of the universe? blasphemy! recant, or die!!!"
i wouldn't mind reading some of the old original ideas. but even newton was influenced a lot by religion with some of his stuff, its cool how people can come up with things that go totally against what is set in stone so to speak and stick by them, and then get proved right later hehe. how long did it take for the catholic church to accept galileos ideas? i think it was a few hundred years for anything official?

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