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If you are working with hardware, yes.
If you have only one midi port, you can address 16 channels on it. For each port you add, 16 new channels can be addressed.
Let's give a simple example. Imagine you have three synths. I'll take multitimbral synths to make things easier (they can listen to several channels at the same time, yes that's possible). We'll keep it simple ok. Let's assume the two first synths "need" 8 channels each. You can connect them both the the first midi port. The third synth will connect to the second midi port, and let's say it only listens to channel 1. For the sake of clarity we'll add a softsynth.
If your hardware is installed right, you should see several options in the midi output routing of the midi track (I assume you know cubase well enough to know what I'm talking about), in our case : midi port A, midi port B and the softsynth.
Lets just see what happens when we only use midi channel 1.
If in the midi out, midi port 1 is selected, the synth that is connected to port A and uses the first 8 channels (remember, we configured it that way) will play. If you select midi port B, the synth connected to port B will play. If you select the softsynth instance, only the softsynth will play.
Let's assume you use midi channel 10. When selecting port A, the second synth will play (because it's connected to port a and listens to channels 9-16). If you select port B, nothing will play because there is no device that listens to channel 9 on port B. If you select the softsynth, it will play, if you set it up right.
I hope this clears things up. Adding a midi port, will add extra channels (input or output, depending on the interface). But you won't always see it as an increase in channel numbers (like midi channel 24 or something like that), that depends on how your sequencer handles that. Most of the time you'll still have 16 channels, but you have to choose which channel, on which midi out.
As for the softsynth part, you can picture it this way. Each instance you create can be seen as a complete synth that comes with it's own midi port.
Working with softsynths is quite simple. Once you start working with a decent amount of midi hardware it starts to get harder (especially if using one master keyboard, but you still want some of the synths to control parameters in your sequencer).
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